Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 cell line
cell type
MCF7 cell line
antibody
ER
treatment
Estrogen +

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed as previously described (Komata, M., et al., Chromatin immunoprecipitation protocol for mammalian cells. Methods Mol Biol, 2014. 1164: p. 33-8.). Total RNA was extracted using TRIzol (Life Technologies) and Nucleospin RNA (Macherey-Nagel) following the manufacturers' instructions. ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
15450131
Reads aligned (%)
94.0
Duplicates removed (%)
25.0
Number of peaks
45119 (qval < 1E-05)

hg19

Number of total reads
15450131
Reads aligned (%)
93.5
Duplicates removed (%)
25.4
Number of peaks
45053 (qval < 1E-05)

Base call quality data from DBCLS SRA